Review





Similar Products

isotype control antibody, mouse igg2a  (Miltenyi Biotec)
95
Miltenyi Biotec isotype control antibody, mouse igg2a
Isotype Control Antibody, Mouse Igg2a, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2a+isotype+control/Isotype+Control+Antibody%2C+mouse+IgG2a/custom%40130-113-833%4042727576
Average 95 stars, based on 1 article reviews
isotype control antibody, mouse igg2a - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
rat igg2a isotype control antibody  (InvivoGen)
94
InvivoGen rat igg2a isotype control antibody
a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
Rat Igg2a Isotype Control Antibody, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2a+isotype+control/Rat+IgG2a+Control/pmc13550523-457-40-48
Average 94 stars, based on 1 article reviews
rat igg2a isotype control antibody - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
igg2a mabs isotype control  (Bio X Cell)
98
Bio X Cell igg2a mabs isotype control
a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
Igg2a Mabs Isotype Control, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2a+isotype+control/InVivoMAb+rat+IgG2a+isotype+control%2C+anti-trinitrophenol/pmc13650256-56-33-42
Average 98 stars, based on 1 article reviews
igg2a mabs isotype control - by Bioz Stars, 2026-10
98/100 stars
  Buy from Supplier
isotype control  (Bio X Cell)
95
Bio X Cell isotype control
a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
Isotype Control, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2a+isotype+control/InVivoMAb+anti-rat+IgG2a/pm42816626-736-14-19
Average 95 stars, based on 1 article reviews
isotype control - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
in house tg2 monoclonal antibody dh2  (R&D Systems)
94
R&D Systems in house tg2 monoclonal antibody dh2
a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
In House Tg2 Monoclonal Antibody Dh2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2a+isotype+control/Mouse+IgG2A+Isotype+Control/pm42779478-107-4-16
Average 94 stars, based on 1 article reviews
in house tg2 monoclonal antibody dh2 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
mouse igg2a  (Bio X Cell)
96
Bio X Cell mouse igg2a
a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
Mouse Igg2a, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2a+isotype+control/InVivoMAb+mouse+IgG2a+isotype+control%2C+unknown+specificity/10__1158_slash_0008___5472__can___26___0770-63-17-21
Average 96 stars, based on 1 article reviews
mouse igg2a - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
isotype control antibody  (Bio X Cell)
98
Bio X Cell isotype control antibody
a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
Isotype Control Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2a+isotype+control/InVivoMAb+rat+IgG2a+isotype+control%2C+anti-trinitrophenol/10__1016_slash_j__gendis__2026__102441-412-21-30
Average 98 stars, based on 1 article reviews
isotype control antibody - by Bioz Stars, 2026-10
98/100 stars
  Buy from Supplier
igg2a isotype control  (Bio X Cell)
96
Bio X Cell igg2a isotype control
a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
Igg2a Isotype Control, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2a+isotype+control/InVivoMAb+mouse+IgG2a+isotype+control%2C+unknown+specificity/10__1038_slash_s41523___026___01016___w-252-29-36
Average 96 stars, based on 1 article reviews
igg2a isotype control - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
rat igg2a isotype control antibody  (Bio X Cell)
98
Bio X Cell rat igg2a isotype control antibody
a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
Rat Igg2a Isotype Control Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2a+isotype+control/InVivoMAb+rat+IgG2a+isotype+control%2C+anti-trinitrophenol/pm42526438-544-13-20
Average 98 stars, based on 1 article reviews
rat igg2a isotype control antibody - by Bioz Stars, 2026-10
98/100 stars
  Buy from Supplier

Image Search Results


a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat IgG2a Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.

Journal: Nature Communications

Article Title: MALT1 protease inhibition restrains glioblastoma progression by reversing tumor-associated macrophage-dependent immunosuppression in mice

doi: 10.1038/s41467-026-76572-7

Figure Lengend Snippet: a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat IgG2a Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.

Article Snippet: In experiments with the MALT1 inhibitor, macrophages were exposed to MLT-748 (5 μM, catalog no.: HY-115466, Med Chem Express), BRD5529 (50 μM, catalog no.: HY-115497), antibody against murine Dectin-1 (clone R1-8g7) (1–5 μg/mL, catalog no.: mabg-mdect-2, Invivogen) or the appropriate Rat IgG2a Isotype Control Antibody (catalog no.: mabg2a-ctlrt, Invivogen) for 2 h prior to addition of tumor cells and during the entire co-culture time.

Techniques: Activity Assay, Stable Transfection, Expressing, Luciferase, Activation Assay, Cell Culture, Control, Co-Culture Assay, Blocking Assay, Cell Analysis